Analysis of fabric evolution and metamorphic reaction progress at Lago della Vecchia-Valle d'Irogna, Sesia-Lanzo Zone, Western Alps

The Lago della Vecchia-Valle d'Irogna rocks are part of the Eclogitic Micaschists Complex (EMC) of the Sesia-Lanzo Zone, western Austroalpine domain. The 1:10,000 scale map includes metaintrusive, minor micaschist, banded gneiss, and metabasic boudins. The multiscale structural analysis reveals successive magmatic and tectono-metamorphic stages: during M0 the metaintrusive protoliths emplaced; D1 took place under eclogite-facies conditions; during D2 stage, a pervasive foliation developed under retrograde blueschist-facies conditions; D3–D4 and D5 structures developed under greenschist-facies conditions; during M6 andesitic dykes intruded. The mapped degree of fabric evolution (FE) and metamorphic transformation (MT) related to D2-foliation shows that the MT was not only controlled by bulk rock and mineral compositions, but also by FE. The development of a pervasive blueschist-facies D2-foliation is in contrast with the eclogitic dominant fabric generally recorded in the EMC. This difference suggests that FE and MT are potentially responsible for km-scale heterogeneities in the tectono-metamorphic record

Enhancement of Heavy-Duty Engines Performance and Reliability Using Cylinder Pressure Information

Sustainability issues are becoming increasingly prominent in applications requiring the use of heavy-duty engines. Therefore, it is important to cut emissions and costs of such engines to re-duce the carbon footprint and keep the operating expenses under control. Even if for some applications a battery electric equipment is introduced, the diesel-equipped machinery is still popular, thanks to the longer operating range. In this field, the open pit mines are a good example. In fact, the Total Cost of Ownership (TCO) of the mining equipment is highly impacted by fuel consumption (engine efficiency) and reliability (service interval and en-gine life). The present work is focused on efficiency enhancements achievable through the ap-plication of a combustion control strategy based on the in-cylinder pressure information. The benefits are mainly due to two factors. First, the negative effects of injectors ageing can be com-pensated. Second, cylindrical online calibration of the control parameters enables the combus-tion system optimization. The article is divided into two parts. The first part describes the tool-chain that is designed for the real time application of the combustion control system, while the second part concerns the algorithm that would be implemented on the Engine Control Unit (ECU) to leverage the in-cylinder pressure information. The assessment of the potential benefits and feasibility of the combustion control algorithm is carried out in a Software in the Loop (SiL) environment, simulating both the developed control strategy and the engine behavior (Liebherr D98). Our goal is to validate the control algorithm through SiL simulations. The results of the validation process demonstrate the effectiveness of the control strategy: firstly, cylinder dispari-ty on IMEP (+/-2.5% in reference conditions) is virtually canceled. Secondly, MFB50 is individual-ly optimized, equalizing Pmax among the cylinders (+/-4% for the standard calibration), without exceeding the reliability threshold. In addition to this, BSFC is reduced by 1%, thanks to the ac-curate cylinder-by-cylinder calibration. Finally, ageing effects or fuel variations can be implicitly compensated, keeping optimal performance thorough engine life

Comparison of Knock Indexes Based on CFD Analysis

Abstract Recent trends in gasoline engines, such as downsizing, downspeeding and the increase of the compression ratio make knocking combustions a serious limiting factor for engine performance. A detailed analysis of knocking events can help improving the engine performance and diagnostic strategies. An effective way is to use advanced 3D Computational Fluid Dynamics (CFD) simulation for the analysis and prediction of the combustion process. The effects of Cycle to Cycle Variation (CCV) on knocking combustions are taken into account, maintaining a \RANS\ (Reynolds Averaged Navier-Stokes) \CFD\ approach, while representing a complex running condition, where knock intensity changes from cycle to cycle. The focus of the numerical methodology is the statistical evaluation of the local air-to-fuel and turbulence distribution at the spark plugs and their correlation with the variability of the initial stages of combustion. \CFD\ simulations have been used to reproduce knock effect on the cylinder pressure trace. For this purpose, the \CFD\ model has been validated, proving its ability to predict the combustion evolution with respect to \SA\ variations, from non-knocking up to heavy knocking conditions. The pressure traces simulated by the \CFD\ model are then used to evaluate cylinder pressure-based knock indexes. Since the model is able to output other knock intensity tracers, such as the mass of fuel burned in knocking mode, or the local heat transferred to the piston, knock indexes based on the cylinder pressure trace can be related to parameters only available in a simulation environment, that are likely to be more representative of the actual knock intensity, with respect to the local pressure trace for the sensor position. The possibility of simulating hundredths of engine cycle allows using the methodology to compare the indexes quality (correlation with actual knock intensity) on a statistical base

Accelerometer Based Methodology for Combustion Parameters Estimation

Due to increasingly stringent emission regulations and the need of more efficient powertrains, obtaining information about combustion process becomes a key factor. Low-cost in-cylinder pressure sensors are being developed, but they still present longterm reliability issues, and represent a considerable part of the engine management system cost. Research is being conducted in order to develop methodologies for extracting relevant combustion information using standard sensors already installed on-board. The present work introduces a methodology for combustion parameters estimation, through a control-oriented analysis of structureborne sound. The paper also shows experimental results obtained applying the estimation methodology to different passenger car engines. © 2015 The Authors. Published by Elsevier Ltd

The W100 pocket on HIV-1 gp120 penetrated by b12 is not a target for other CD4bs monoclonal antibodies

<p>Abstract</p> <p>Background</p> <p>The conserved CD4 binding site (CD4bs) on HIV-1 gp120 is a major target for vaccines. It is a priority to determine sites and structures within the CD4bs that are important for inclusion in vaccines. We studied a gp120 pocket penetrated by W100 of the potent CD4bs monoclonal antibody (mab), b12. We compared HIV-1 envelopes and corresponding mutants that carried blocked W100 pockets to evaluate whether other CD4bs mabs target this site.</p> <p>Findings</p> <p>All CD4bs mabs tested blocked soluble CD4 binding to gp120 consistent with their designation as CD4bs directed antibodies. All CD4bs mabs tested neutralized pseudovirions carrying NL4.3 wild type (wt) envelope. However, only b12 failed to neutralize pseudoviruses carrying mutant envelopes with a blocked W100 pocket. In addition, for CD4bs mabs that neutralized pseudovirions carrying primary envelopes, mutation of the W100 pocket had little or no effect on neutralization sensitivity.</p> <p>Conclusions</p> <p>Our data indicate that the b12 W100 pocket on gp120 is infrequently targeted by CD4bs mabs. This site is therefore not a priority for preservation in vaccines aiming to elicit antibodies targeting the CD4bs.</p

Escape from Human Monoclonal Antibody Neutralization Affects In Vitro and In Vivo Fitness of Severe Acute Respiratory Syndrome Coronavirus

Severe Acute Respiratory Syndrome (SARS) emerged as a human disease in 2002 and detailed phylogenetic analysis and epidemiological studies have suggested that the SARS-Coronavirus (SARS-CoV) originated from animals. The Spike (S) glycoprotein has been identified as a major target of protective immunity and contains at least three regions that are targeted by neutralizing antibodies in the S1 and S2 domains. We previously characterized a panel of neutralizing human monoclonal antibodies (MAbs) but the majority of epitopes recognized by the MAbs remained unknown

Transient Spark Advance Calibration Approach

Combustion control is assuming a crucial role in reducing engine tailpipe emissions while maximizing performance. The effort in the calibration of control parameters affecting the combustion development can be very demanding. One of the most effective factors influencing performance and efficiency is the combustion phasing: in Spark Ignition (SI) engines it is affected by factors such as Spark Advance (SA), Air-Fuel Ratio (AFR), Exhaust Gas Recirculation (EGR), Variable Valve Timing (VVT). SA optimal values are usually determined by means of calibration procedures carried out in steady state conditions on the test bench by changing SA values while monitoring performance indicators, such as Brake and Indicated Mean Effective Pressure (BMEP, IMEP), Brake Specific Fuel Consumption (BSFC) and pollutant emissions. The effect of SA on combustion is stochastic, due to the cycle-to-cycle variation: the analysis of mean values requires many engine cycles to be significant of the performance obtained with the given control setting. Moreover, often the effect of SA on engine performance must be investigated for different settings of other control parameters (EGR, VVT, AFR). The calibration process is time consuming involving exhaustive tests followed by off-line data analysis. This paper presents the application of a dynamic calibration methodology, with the objective of reducing the calibration duration. The proposed approach is based on transient tests, coupled with a statistical investigation, allowing reliable performance analysis even with a low number of engine cycles. The methodology has been developed and tested off-line, then it has been implemented in Real-Time. The combustion analysis system has been integrated with the ECU management software and the test bench controller, in order to perform a fully automatic calibration. © 2013 The Authors

Immunogenetic Mechanisms Driving Norovirus GII.4 Antigenic Variation

Noroviruses are the principal cause of epidemic gastroenteritis worldwide with GII.4 strains accounting for 80% of infections. The major capsid protein of GII.4 strains is evolving rapidly, resulting in new epidemic strains with altered antigenic potentials. To test if antigenic drift may contribute to GII.4 persistence, human memory B cells were immortalized and the resulting human monoclonal antibodies (mAbs) characterized for reactivity to a panel of time-ordered GII.4 virus-like particles (VLPs). Reflecting the complex exposure history of the volunteer, human anti-GII.4 mAbs grouped into three VLP reactivity patterns; ancestral (1987–1997), contemporary (2004–2009), and broad (1987–2009). NVB 114 reacted exclusively to the earliest GII.4 VLPs by EIA and blockade. NVB 97 specifically bound and blocked only contemporary GII.4 VLPs, while NBV 111 and 43.9 exclusively reacted with and blocked variants of the GII.4.2006 Minerva strain. Three mAbs had broad GII.4 reactivity. Two, NVB 37.10 and 61.3, also detected other genogroup II VLPs by EIA but did not block any VLP interactions with carbohydrate ligands. NVB 71.4 cross-neutralized the panel of time-ordered GII.4 VLPs, as measured by VLP-carbohydrate blockade assays. Using mutant VLPs designed to alter predicted antigenic epitopes, two evolving, GII.4-specific, blockade epitopes were mapped. Amino acids 294–298 and 368–372 were required for binding NVB 114, 111 and 43.9 mAbs. Amino acids 393–395 were essential for binding NVB 97, supporting earlier correlations between antibody blockade escape and carbohydrate binding variation. These data inform VLP vaccine design, provide a strategy for expanding the cross-blockade potential of chimeric VLP vaccines, and identify an antibody with broadly neutralizing therapeutic potential for the treatment of human disease. Moreover, these data support the hypothesis that GII.4 norovirus evolution is heavily influenced by antigenic variation of neutralizing epitopes and consequently, antibody-driven receptor switching; thus, protective herd immunity is a driving force in norovirus molecular evolution

Stress and wellbeing among professionals working with people with neurodevelopmental disorders. Review and intervention perspectives

AbstractSupporting individuals with NDD is extremely demanding, with significant exposure tocritical contexts and events, and painful ongoing experiences. Stress and burnout conditionis a main concern with growing interest in research, despite the lack of consensus ontheoretical explanatory models and modification standards.The paper provides an up-to-date review of risk factors and involved processes, and presentsevidence-based procedures and protocols to implement effective preventive actionsaddressing both organizational and individual factors. The aim is to offer a global understandingof the subject and offer examples of practical plans to increase the impact onthe quality of life of clients and staff members